PCR exponentially multiplies the available piece of nucleic acid to such an extent that it can be measured, although at the beginning that piece may have been in very small concentration. This is a basis for the particularly good sensitivity of this method.
Primers are synthesized at a low cost, making this analytical method one of the cheapest. If you have added cheap fish or cheap meat to your food, then this method can relatively, cheaply and quickly detect that fact. If primer sequences are not available, you can create them yourself for free.
You can find the gene of interest in the NCBI Gene database. Take Accession from there and transfer this number to Primer-BLAST, found here: https://www.ncbi.nlm.nih.gov/tools/primer-blast/. They will provide default settings that you can try. If we are working with ribonucleic acid, it is worth specifying that the product will include an exon junction (in the Intron inclusion column it should be noted: Primer pair must be separated by at least one intron on the corresponding genomic DNA) so that the problems of DNA contamination are not a concern. You can recover many options. We prefer primers that receive ~ 200 bp of product (this can be written in the PCR product size line: Max write 200) and have minimal unintended effects.
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